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calbindin d28k guinea pig polyclonal igg antibody  (Synaptic Systems)


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    Synaptic Systems calbindin d28k guinea pig polyclonal igg antibody
    The impact of burn injury on cortical neuronal activity: c-Fos immunoreactivity (IR) analysis. ( a ) Schematic representation illustrating the time scale of this experiment. ( b ) Schematic drawing of a coronal section of the mouse brain representing the lower limb area of the S1 cortex. ( c ) Representative immunostaining with an antibody against c-Fos (red) was obtained from three optical sections of a coronal slice of a wild-type mouse brain following burn injury. DAPI counterstained cell nuclei for turquoise. After the burn injury, the number of cells exhibiting c-Fos-IR increased in the lower limb area of the S1 cortex, irrespective of the sides. The term “burn injury-contralateral” specifically refers to the right hemisphere of the brain, which is opposite the burn injury site. Scale bar, 220 μm. ( d ) Quantitative analysis of the percentage of c-Fos-IR nuclei in the lower limb area of the S1 cortex following burn injury. The data are presented as mean ± SEM (10–11 slices from 4 animals per group). An asterisk denotes a p -value < 0.05, while a double asterisk indicates a p -value < 0.01, vs. control. ( e ) Quantitative analysis of the percentage of colocalization between c-Fos and various interneuron subtypes in the S1 cortex following burn injury. The data are presented as mean ± SEM (5–11 slices from 2–4 animals per group). * signifies p < 0.05, while ** indicates p < 0.01, vs. control. ( f ) Representative triple immunostaining with antibodies against c-Fos, parvalbumin (Pvalb), and calretinin (CR) from layer 2/3 of the contralateral-sided S1 cortex following burn injury. Arrowhead shows a CR-positive interneuron that expresses c-Fos. Scale bar, 30 μm. ( g ) Representative triple immunostaining with antibodies against c-Fos, <t>calbindin</t> (Calb), and a nuclear marker, DAPI, from layer 2/3 of the contralateral-sided S1 cortex following burn injury. The dotted line indicates the boundary between layer 1 and layer 2/3. Arrowheads show colocalizations of c-Fos and Calb. Scale bar, 30 μm. Abbreviations: WT, wild type; PFA, paraformaldehyde; S1, primary somatosensory cortex; MOp, primary motor cortex; V3, third ventricle; IC, internal capsule; LV, lateral ventricle; CC, corpus callosum.
    Calbindin D28k Guinea Pig Polyclonal Igg Antibody, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calbindin+d28k+guinea+pig+polyclonal+igg+antibody/guinea+pig+anti+calbindin/pmc12027419-3-0-10
    Average 90 stars, based on 1 article reviews
    calbindin d28k guinea pig polyclonal igg antibody - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "The Burning Pain Transcriptome in the Mouse Primary Somatosensory Cortex"

    Article Title: The Burning Pain Transcriptome in the Mouse Primary Somatosensory Cortex

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms26083538

    The impact of burn injury on cortical neuronal activity: c-Fos immunoreactivity (IR) analysis. ( a ) Schematic representation illustrating the time scale of this experiment. ( b ) Schematic drawing of a coronal section of the mouse brain representing the lower limb area of the S1 cortex. ( c ) Representative immunostaining with an antibody against c-Fos (red) was obtained from three optical sections of a coronal slice of a wild-type mouse brain following burn injury. DAPI counterstained cell nuclei for turquoise. After the burn injury, the number of cells exhibiting c-Fos-IR increased in the lower limb area of the S1 cortex, irrespective of the sides. The term “burn injury-contralateral” specifically refers to the right hemisphere of the brain, which is opposite the burn injury site. Scale bar, 220 μm. ( d ) Quantitative analysis of the percentage of c-Fos-IR nuclei in the lower limb area of the S1 cortex following burn injury. The data are presented as mean ± SEM (10–11 slices from 4 animals per group). An asterisk denotes a p -value < 0.05, while a double asterisk indicates a p -value < 0.01, vs. control. ( e ) Quantitative analysis of the percentage of colocalization between c-Fos and various interneuron subtypes in the S1 cortex following burn injury. The data are presented as mean ± SEM (5–11 slices from 2–4 animals per group). * signifies p < 0.05, while ** indicates p < 0.01, vs. control. ( f ) Representative triple immunostaining with antibodies against c-Fos, parvalbumin (Pvalb), and calretinin (CR) from layer 2/3 of the contralateral-sided S1 cortex following burn injury. Arrowhead shows a CR-positive interneuron that expresses c-Fos. Scale bar, 30 μm. ( g ) Representative triple immunostaining with antibodies against c-Fos, calbindin (Calb), and a nuclear marker, DAPI, from layer 2/3 of the contralateral-sided S1 cortex following burn injury. The dotted line indicates the boundary between layer 1 and layer 2/3. Arrowheads show colocalizations of c-Fos and Calb. Scale bar, 30 μm. Abbreviations: WT, wild type; PFA, paraformaldehyde; S1, primary somatosensory cortex; MOp, primary motor cortex; V3, third ventricle; IC, internal capsule; LV, lateral ventricle; CC, corpus callosum.
    Figure Legend Snippet: The impact of burn injury on cortical neuronal activity: c-Fos immunoreactivity (IR) analysis. ( a ) Schematic representation illustrating the time scale of this experiment. ( b ) Schematic drawing of a coronal section of the mouse brain representing the lower limb area of the S1 cortex. ( c ) Representative immunostaining with an antibody against c-Fos (red) was obtained from three optical sections of a coronal slice of a wild-type mouse brain following burn injury. DAPI counterstained cell nuclei for turquoise. After the burn injury, the number of cells exhibiting c-Fos-IR increased in the lower limb area of the S1 cortex, irrespective of the sides. The term “burn injury-contralateral” specifically refers to the right hemisphere of the brain, which is opposite the burn injury site. Scale bar, 220 μm. ( d ) Quantitative analysis of the percentage of c-Fos-IR nuclei in the lower limb area of the S1 cortex following burn injury. The data are presented as mean ± SEM (10–11 slices from 4 animals per group). An asterisk denotes a p -value < 0.05, while a double asterisk indicates a p -value < 0.01, vs. control. ( e ) Quantitative analysis of the percentage of colocalization between c-Fos and various interneuron subtypes in the S1 cortex following burn injury. The data are presented as mean ± SEM (5–11 slices from 2–4 animals per group). * signifies p < 0.05, while ** indicates p < 0.01, vs. control. ( f ) Representative triple immunostaining with antibodies against c-Fos, parvalbumin (Pvalb), and calretinin (CR) from layer 2/3 of the contralateral-sided S1 cortex following burn injury. Arrowhead shows a CR-positive interneuron that expresses c-Fos. Scale bar, 30 μm. ( g ) Representative triple immunostaining with antibodies against c-Fos, calbindin (Calb), and a nuclear marker, DAPI, from layer 2/3 of the contralateral-sided S1 cortex following burn injury. The dotted line indicates the boundary between layer 1 and layer 2/3. Arrowheads show colocalizations of c-Fos and Calb. Scale bar, 30 μm. Abbreviations: WT, wild type; PFA, paraformaldehyde; S1, primary somatosensory cortex; MOp, primary motor cortex; V3, third ventricle; IC, internal capsule; LV, lateral ventricle; CC, corpus callosum.

    Techniques Used: Activity Assay, Immunostaining, Control, Triple Immunostaining, Marker

    List of the primary antibodies used in the study, including their suppliers, catalog numbers, and dilution factors.
    Figure Legend Snippet: List of the primary antibodies used in the study, including their suppliers, catalog numbers, and dilution factors.

    Techniques Used:

    Related Articles

    Activity Assay:

    Article Title: The Burning Pain Transcriptome in the Mouse Primary Somatosensory Cortex
    Article Snippet: calbindin D28k , guinea pig polyclonal IgG , 1:250 , Synaptic Systems GmbH.; Göttingen, Germany , 214004.

    Immunostaining:

    Article Title: The Burning Pain Transcriptome in the Mouse Primary Somatosensory Cortex
    Article Snippet: calbindin D28k , guinea pig polyclonal IgG , 1:250 , Synaptic Systems GmbH.; Göttingen, Germany , 214004.

    Control:

    Article Title: The Burning Pain Transcriptome in the Mouse Primary Somatosensory Cortex
    Article Snippet: calbindin D28k , guinea pig polyclonal IgG , 1:250 , Synaptic Systems GmbH.; Göttingen, Germany , 214004.

    Triple Immunostaining:

    Article Title: The Burning Pain Transcriptome in the Mouse Primary Somatosensory Cortex
    Article Snippet: calbindin D28k , guinea pig polyclonal IgG , 1:250 , Synaptic Systems GmbH.; Göttingen, Germany , 214004.

    Marker:

    Article Title: The Burning Pain Transcriptome in the Mouse Primary Somatosensory Cortex
    Article Snippet: calbindin D28k , guinea pig polyclonal IgG , 1:250 , Synaptic Systems GmbH.; Göttingen, Germany , 214004.



    Similar Products

    calbindin d28k guinea pig polyclonal igg antibody  (Synaptic Systems)
    90
    Synaptic Systems calbindin d28k guinea pig polyclonal igg antibody
    The impact of burn injury on cortical neuronal activity: c-Fos immunoreactivity (IR) analysis. ( a ) Schematic representation illustrating the time scale of this experiment. ( b ) Schematic drawing of a coronal section of the mouse brain representing the lower limb area of the S1 cortex. ( c ) Representative immunostaining with an antibody against c-Fos (red) was obtained from three optical sections of a coronal slice of a wild-type mouse brain following burn injury. DAPI counterstained cell nuclei for turquoise. After the burn injury, the number of cells exhibiting c-Fos-IR increased in the lower limb area of the S1 cortex, irrespective of the sides. The term “burn injury-contralateral” specifically refers to the right hemisphere of the brain, which is opposite the burn injury site. Scale bar, 220 μm. ( d ) Quantitative analysis of the percentage of c-Fos-IR nuclei in the lower limb area of the S1 cortex following burn injury. The data are presented as mean ± SEM (10–11 slices from 4 animals per group). An asterisk denotes a p -value < 0.05, while a double asterisk indicates a p -value < 0.01, vs. control. ( e ) Quantitative analysis of the percentage of colocalization between c-Fos and various interneuron subtypes in the S1 cortex following burn injury. The data are presented as mean ± SEM (5–11 slices from 2–4 animals per group). * signifies p < 0.05, while ** indicates p < 0.01, vs. control. ( f ) Representative triple immunostaining with antibodies against c-Fos, parvalbumin (Pvalb), and calretinin (CR) from layer 2/3 of the contralateral-sided S1 cortex following burn injury. Arrowhead shows a CR-positive interneuron that expresses c-Fos. Scale bar, 30 μm. ( g ) Representative triple immunostaining with antibodies against c-Fos, <t>calbindin</t> (Calb), and a nuclear marker, DAPI, from layer 2/3 of the contralateral-sided S1 cortex following burn injury. The dotted line indicates the boundary between layer 1 and layer 2/3. Arrowheads show colocalizations of c-Fos and Calb. Scale bar, 30 μm. Abbreviations: WT, wild type; PFA, paraformaldehyde; S1, primary somatosensory cortex; MOp, primary motor cortex; V3, third ventricle; IC, internal capsule; LV, lateral ventricle; CC, corpus callosum.
    Calbindin D28k Guinea Pig Polyclonal Igg Antibody, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calbindin+d28k+guinea+pig+polyclonal+igg+antibody/guinea+pig+anti+calbindin/pmc12027419-3-0-10
    Average 90 stars, based on 1 article reviews
    calbindin d28k guinea pig polyclonal igg antibody - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    The impact of burn injury on cortical neuronal activity: c-Fos immunoreactivity (IR) analysis. ( a ) Schematic representation illustrating the time scale of this experiment. ( b ) Schematic drawing of a coronal section of the mouse brain representing the lower limb area of the S1 cortex. ( c ) Representative immunostaining with an antibody against c-Fos (red) was obtained from three optical sections of a coronal slice of a wild-type mouse brain following burn injury. DAPI counterstained cell nuclei for turquoise. After the burn injury, the number of cells exhibiting c-Fos-IR increased in the lower limb area of the S1 cortex, irrespective of the sides. The term “burn injury-contralateral” specifically refers to the right hemisphere of the brain, which is opposite the burn injury site. Scale bar, 220 μm. ( d ) Quantitative analysis of the percentage of c-Fos-IR nuclei in the lower limb area of the S1 cortex following burn injury. The data are presented as mean ± SEM (10–11 slices from 4 animals per group). An asterisk denotes a p -value < 0.05, while a double asterisk indicates a p -value < 0.01, vs. control. ( e ) Quantitative analysis of the percentage of colocalization between c-Fos and various interneuron subtypes in the S1 cortex following burn injury. The data are presented as mean ± SEM (5–11 slices from 2–4 animals per group). * signifies p < 0.05, while ** indicates p < 0.01, vs. control. ( f ) Representative triple immunostaining with antibodies against c-Fos, parvalbumin (Pvalb), and calretinin (CR) from layer 2/3 of the contralateral-sided S1 cortex following burn injury. Arrowhead shows a CR-positive interneuron that expresses c-Fos. Scale bar, 30 μm. ( g ) Representative triple immunostaining with antibodies against c-Fos, calbindin (Calb), and a nuclear marker, DAPI, from layer 2/3 of the contralateral-sided S1 cortex following burn injury. The dotted line indicates the boundary between layer 1 and layer 2/3. Arrowheads show colocalizations of c-Fos and Calb. Scale bar, 30 μm. Abbreviations: WT, wild type; PFA, paraformaldehyde; S1, primary somatosensory cortex; MOp, primary motor cortex; V3, third ventricle; IC, internal capsule; LV, lateral ventricle; CC, corpus callosum.

    Journal: International Journal of Molecular Sciences

    Article Title: The Burning Pain Transcriptome in the Mouse Primary Somatosensory Cortex

    doi: 10.3390/ijms26083538

    Figure Lengend Snippet: The impact of burn injury on cortical neuronal activity: c-Fos immunoreactivity (IR) analysis. ( a ) Schematic representation illustrating the time scale of this experiment. ( b ) Schematic drawing of a coronal section of the mouse brain representing the lower limb area of the S1 cortex. ( c ) Representative immunostaining with an antibody against c-Fos (red) was obtained from three optical sections of a coronal slice of a wild-type mouse brain following burn injury. DAPI counterstained cell nuclei for turquoise. After the burn injury, the number of cells exhibiting c-Fos-IR increased in the lower limb area of the S1 cortex, irrespective of the sides. The term “burn injury-contralateral” specifically refers to the right hemisphere of the brain, which is opposite the burn injury site. Scale bar, 220 μm. ( d ) Quantitative analysis of the percentage of c-Fos-IR nuclei in the lower limb area of the S1 cortex following burn injury. The data are presented as mean ± SEM (10–11 slices from 4 animals per group). An asterisk denotes a p -value < 0.05, while a double asterisk indicates a p -value < 0.01, vs. control. ( e ) Quantitative analysis of the percentage of colocalization between c-Fos and various interneuron subtypes in the S1 cortex following burn injury. The data are presented as mean ± SEM (5–11 slices from 2–4 animals per group). * signifies p < 0.05, while ** indicates p < 0.01, vs. control. ( f ) Representative triple immunostaining with antibodies against c-Fos, parvalbumin (Pvalb), and calretinin (CR) from layer 2/3 of the contralateral-sided S1 cortex following burn injury. Arrowhead shows a CR-positive interneuron that expresses c-Fos. Scale bar, 30 μm. ( g ) Representative triple immunostaining with antibodies against c-Fos, calbindin (Calb), and a nuclear marker, DAPI, from layer 2/3 of the contralateral-sided S1 cortex following burn injury. The dotted line indicates the boundary between layer 1 and layer 2/3. Arrowheads show colocalizations of c-Fos and Calb. Scale bar, 30 μm. Abbreviations: WT, wild type; PFA, paraformaldehyde; S1, primary somatosensory cortex; MOp, primary motor cortex; V3, third ventricle; IC, internal capsule; LV, lateral ventricle; CC, corpus callosum.

    Article Snippet: calbindin D28k , guinea pig polyclonal IgG , 1:250 , Synaptic Systems GmbH.; Göttingen, Germany , 214004.

    Techniques: Activity Assay, Immunostaining, Control, Triple Immunostaining, Marker

    List of the primary antibodies used in the study, including their suppliers, catalog numbers, and dilution factors.

    Journal: International Journal of Molecular Sciences

    Article Title: The Burning Pain Transcriptome in the Mouse Primary Somatosensory Cortex

    doi: 10.3390/ijms26083538

    Figure Lengend Snippet: List of the primary antibodies used in the study, including their suppliers, catalog numbers, and dilution factors.

    Article Snippet: calbindin D28k , guinea pig polyclonal IgG , 1:250 , Synaptic Systems GmbH.; Göttingen, Germany , 214004.

    Techniques: